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Based on indo sumatran kratom these observations two possibilities are considered: 1) the effect is cell cycle arrest independent of p53 and p21 pathway or 2) the loss of these proteins could be due to the leakage due to the increased membrane permeability or through pore opening. The toxicity findings noted thus far are consistent with my hypothesis in which the dose is the main factor in determining the level of the cytotoxicity seen. The cytotoxicity events initially seen as cell cycle arrest proceed to cell death with increasing doses of MSE and MIT.

B MIT Treatment without S9 (24 hr) Neg. C 30 20 10 5 MMS Cell conc.
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Relative suspension growth (RSG) 100. Summary table of MLA result for MIT in the i) presence of rat liver S9 and ii) in the absence of rat liver S9. S9 treatment Treatment groups Negative control 0 0 0 30 20 MIT 10 5 Positive control (DMBA) Mean Control MF 76. Negative Negative Negative Negative Negative Negative Negative Best Legal Highs Kratom Positive Conc.

M MIT indicating the loss of p53 protein over time. The findings described above Best Legal Highs Kratom suggest that the cell cycle arrest of MSE treated cells seen previously with flow cytometry was independent of p53 protein induction and to the lesser extent for MIT treated cells. P53 lucky kratom 40 pack levels of MSE treated SH-SY5Y cells after 24 hr treatment.

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